Targeted methylation sequencing (Methyl-Seq) offers a balanced, cost-effective choice between whole-genome bisulfite sequencing and methylation arrays that can support both screening and biomarker discovery study objectives.1 TruSeq Methyl Capture EPIC Library Prep supports researchers seeking a deeper understanding of the role of methylation in gene regulation.
Comprehensive methylation-site content: Offers significantly higher coverage of emerging epigenetic regions of interest compared to other Methyl-Seq kits currently on the market (See Supporting Data section, Epigenetic Regions Covered).
Maximize discovery power, minimize cost: Efficiently query differentially methylated bases to conserve research dollars.
Convenient kit configuration: Includes bisulfite conversion, library prep, target enrichment, and purification reagents in a single kit for hassle-free ordering.
User-friendly data analysis: Analyze sequence data in BaseSpace Sequence Hub with methylation apps designed for biologists.
Instrument | Recommended Number of Samples | Read Length |
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NextSeq 550 System | 8 samples per run (high output; based on 40× mean coverage and >90% of target bases covered at ≥10×) | 2 x 100 bp (max recommended) |
NovaSeq 6000 System | Samples per run (dual flow cell): S1: 48, S2: 48, S4: 96 (limited by available index combinations; based on >55 million reads) | 2 × 100 bp (max recommended) |
HiSeq 3000 System | 48 samples per run (based on 40× mean coverage and >90% of target bases covered at ≥10×) | 2 × 100 bp (max recommended) |
HiSeq 4000 System | 96 samples per run (based on 40× mean coverage and >90% of target bases covered at ≥10×) | 2 × 100 bp (max recommended) |
HiSeq 4000 System | 96 samples per run (based on 40× mean coverage and >90% of target bases covered at ≥10×) | 2 × 100 bp (max recommended) |
TruSeq Methyl Capture EPIC Library Prep Kit | Infinium MethylationEPIC v2.0 Kit | |
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Content Specifications | Targets over 3.3 million CpGs. Interrogates biologically important methylome targets. | |
Description | Focused interrogation of a set of methylome targets for a balanced, cost-effective option that can support both screening studies and biomarker discovery research objectives. | Quantitative interrogation of selected methylation sites across the genome, offering high-throughput research capabilities at a minimal cost per sample. |
Input Quantity | 500 ng human genomic DNA | 250 ng DNA |
Method | Methylation Sequencing, Targeted DNA Sequencing | Methylation Array |
Number of Samples | Up to 12-plex | 8 samples per array |
Species Category | Human | Human |
Technology | Sequencing | Microarray |
TruSeq Methyl Capture EPIC Library Prep Kit
Data Sheet | PDF 2 MB