NovaSeq X Series

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Change the world with the NovaSeq X series

Breakthrough innovations for groundbreaking discoveries

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Maximize throughput to unleash your ambitions

The NovaSeq X and NovaSeq X Plus Sequencing Systems deliver extraordinary throughput and accuracy to perform data-intensive applications at production scale.

Built on decades of innovation from a trusted partner in genomics, the NovaSeq X series will enable your most ambitious ultra-high-throughput projects. These are our most powerful sequencers yet. Scale your studies with three flow cell types and up to 16 Tb output per run on the dual flow cell NovaSeq X Plus system or up to 8 Tb on the single flow cell NovaSeq X system. Ultra-high-density flow cells and ultra-high-resolution optics enable output of up to 26 billion single reads per flow cell.

The NovaSeq X series is powered by XLEAP-SBS chemistry — an even faster, higher quality, and more robust version of our proven sequencing by synthesis (SBS) chemistry.

XLEAP-SBS chemistry delivers the highest level of data accuracy and performance, and the increased stability enables remarkable sustainability benefits.

The DRAGEN Bio-IT Platform, available onboard or in the cloud, provides award-winning accuracy for rapid, easy-to-use, and efficient genomic data analysis.

As integrated into the NovaSeq X series, the DRAGEN platform can run multiple secondary analysis pipelines in parallel. Perform up to four simultaneous applications per flow cell in a single run.

The innovations built into the NovaSeq X and NovaSeq X Plus systems have the power to generate tens of thousands of whole genomes per year. Dream big. NovaSeq X series can make it happen.

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NovaSeq X System with doors open

NovaSeq X series specifications

  • Output Range ~165 Gb - 16 Tb
  • Single reads per run 1.6 billion - 52 billion
  • Read length 2 × 150 bp
  • Run time ~13 hr - 48 hr

Transformative economics for transformative breakthroughs

The NovaSeq X series allows you to maximize your productivity and minimize your turnaround time.

Beyond the significant reduction in cost per Gb and per read, the NovaSeq X series incorporates cost-efficiency throughout the workflow. Streamlined informatics, operational simplicity, sustainability advancements, and world-class support lead to the best total cost of ownership equation.

The ultimate user experience

  • Push-button operation with automated onboard cluster generation and post-run wash
  • Individually addressable flow cell lanes with automated onboard independent lane loading
  • Flexible run planning options, touchless secondary analysis workflows, and automated lossless data compression
  • Thoughtful ergonomic design innovations for the ultimate user experience
Sequence more sustainably than ever

Propel your research forward to impact the world, not the environment. The NovaSeq X and NovaSeq X Plus systems were purpose-designed to be our most sustainable sequencing systems yet.

  • Save time and money with a 90% reduction in packaging weight and waste
  • Maximize storage space with compact cartridges and packaging
  • Save disposal costs with more recyclable plastics and no dry ice or ice packs
Lab scientist standing by NovaSeq X Plus Sequencing System holding the most sustainable packaging from Illumina yet
NovaSeq X series Sequencing System

Unimaginable experiments, made unbelievably cost-efficient

The NovaSeq X series helps moonshot projects become a reality—making affordable what was previously out of reach. Increase statistical power with broader study designs and larger sample cohorts. Maximize read numbers and increase sequencing depth for a higher resolution view that can detect low-frequency signals and variants.

The NovaSeq X Plus system revolutionizes human whole-genome sequencing, with the ability to sequence more than 128 genomes per run. These production-scale sequencing systems can also perform a broad range of data-intensive applications, from single-cell analysis to exome and circulating tumor DNA sequencing, that will unlock the next wave of omics discovery.

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Compare with NovaSeq 6000

  NovaSeq X Plus NovaSeq X NovaSeq 6000
Maximum output per run 16 Tb 8 Tb 6 Tb
Maximum reads per run 52 billion single reads
104 billion paired-end reads
26 billion single reads
52 billion paired-end reads
20 billion single reads
40 billion paired-end reads

Popular Products

NovaSeq X Series Reagent Kits

The power of XLEAP-SBS chemistry combined with flexible, efficient, and sustainable workflows for transformational genomics at scale.

Illumina DNA PCR-Free Prep

A high-performing, fast, and integrated workflow for applications such as whole-genome sequencing or tumor-normal sequencing.

IDT for Illumina DNA/RNA UD Indexes

Sets A, B, C, and D offer up to 384 unique dual indexes, enabling accurate assignment of reads and efficient use of the flow cell.

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Get help deciding if the NovaSeq X series and the benefits of ultra-high-throughput sequencing are right for you as you continue to scale up. Contact us today for more information.

Speak to a Specialist