Illumina DNA Prep with Exome 2.0 Plus Enrichment

A high-performance, fast, and complete whole-exome sequencing kit including library prep and hybridization reagents, a comprehensive exome probe panel, clean up/size selection beads, and indexes. Read More...

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Illumina® DNA Prep with Exome 2.0 Plus Enrichment, (S) Tagmentation Set B (96 samples, 12-plex)

20077595

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Illumina® DNA Prep with Exome 2.0 Plus Enrichment, (S) Tagmentation Set D (96 samples, 12-plex)

20077596

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Panel

Twist Bioscience® for Illumina® Mitochondrial Panel (96 samples, 12-plex)

20093180

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Accessory Products

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Flex Lysis Reagent Kit (96 reactions)

20018706

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IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

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IDT® for Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)

20042666

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Infinium MIDI Heatblock Insert

BD-60-601

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Illumina® Free Adapter Blocking Reagent (12 Reactions)

20024144

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Illumina® Free Adapter Blocking Reagent (48 Reactions)

20024145

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Illumina Purification Beads (IPB)

20060057

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Illumina Purification Beads (IPB)

20060058

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Product Highlights

Illumina DNA Prep with Exome 2.0 Plus Enrichment offers a cost-effective, reliable solution for human whole-exome sequencing (WES).

Optional Mitochondrial Genome Enrichment

The Twist Bioscience for Illumina Mitochondrial Panel is a fixed content panel designed to cover the 16,659 base pairs (bp) and 37 genes of the mitochondrial genome (chrM) and allows enrichment and sequencing of mitochondrial DNA (mtDNA) variants. Use this panel as a spike-in with the Twist Bioscience for Illumina Exome 2.0 Plus Panel and add coverage of ChrM to your experiments, with the same high WES on-target rates and coverage uniformity.

Outstanding Enrichment Performance

Illumina DNA Prep with Exome 2.0 Plus Enrichment provides high on-target rates and uniform coverage enabling high multiplexing, in turn decreasing costs and increasing sample throughput.

Automation-friendly Workflow

Prepare libraries for Illumina sequencing in less than a day. Illumina Qualified Methods are available on a range of automation platforms through our partners. To view partner documentation, please visit our partner websites, Eppendorf, Hamilton and PerkinElmer.

Fast, Streamlined Workflow

Illumina DNA Prep with Exome 2.0 Plus Enrichment uses a fast, user-friendly workflow with built-in library yield normalization and a simplified, single hybridization protocol. Go from DNA to library prep, enrichment, and post-enrichment amplification in just 6.5 hours.

Single-Vendor End-to-End Solution

You and your lab can benefit from a WES solution with support from a single, reliable partner—starting from direct blood or saliva input, through library prep, enrichment, sequencing, analysis, interpretation, and reporting.

Frequently Purchased Together

Specifications

Project Recommendations

Instrument	Recommended Number of Samples	Read Length
NextSeq 2000 System	Samples per run (by flow cell type): P2: 19, P3: 57 (based on 50× mean target coverage, 42M reads, 4.2 Gb of data)	2 × 101 bp
NovaSeq 6000 System	Samples per run by flow cell type: SP: 34, S1: 69, S2: 176, S4: 428 based on 50× mean target coverage, 42M reads, 4.2 Gb of data. Note: S4: 428 requires NovaSeq Xp workflow or additional indexes.	2 × 101 bp

Product Comparison

	Illumina DNA Prep with Exome 2.0 Plus Enrichment	Illumina DNA Prep with Enrichment	TruSeq DNA Exome
Assay Time	6.5 hours	~6.5 hours	2.5 days
Content Specifications	Twist Bioscience for Illumina Exome 2.0 Plus Panel 37.5 Mb coding content (≥ 99% of RefSeq, CCDS, ClinVar and ACMG pathogenic/likely pathogenic variants, COSMIC Cancer Gene Census), OMIM Twist Bioscience for Illumina Mitochondrial Panel covers the circular mitochondrial genome ChrM (16,659 bp, 37 genes)	Exome: 45 Mb exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). Custom: 0.5 - 15 Mb genomic content of interest. Fixed panels: Content varies by panel.	Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Description	A fast and easy-to-use library prep with enrichment workflow with a focused enrichment probe panel of up-to-date exome content for cost-effective and reliable human whole-exome sequencing.	A fast, flexible research solution that enables whole-exome, custom, and fixed panel targeted sequencing.	A cost-effective exome research workflow focused on ≥98% of RefSeq, CCDS, and Ensembl coding content.
Hands-On Time	~2 hours	~2 hours	6 hours
Input Quantity	50-1000 ng high-quality genomic DNA (For direct blood and saliva, see reference guide)	10-1000 ng high-quality genomic DNA or 50-1000 ng FFPE DNA. (For blood and saliva, see the reference guide).	100 ng genomic DNA
Mechanism of Action	Bead-linked transposome and hybrid-capture chemistry	Bead-bound transposomes and hybrid-capture chemistry	Mechanical fragmentation (Covaris) and exome enrichment with biotinylated capture probes
Method	Exome Sequencing, Target Enrichment, Target Enrichment, Targeted DNA Sequencing	Custom Sequencing, Exome Sequencing, Target Enrichment, Target Enrichment, Targeted DNA Sequencing	Exome Sequencing
Multiplexing	Pre-enrichment pooling of up to 12-plex is tested. Kit configuration supports multiplexing up to 192 samples. Up to 384 possible.	Up to 384 available unique dual indexes. Pre-enrichment pooling of up to 12-plex is tested.	Up to 12-plex enrichment
Nucleic Acid Type	DNA	DNA	DNA
On-Target Reads	~ 90% (ave.) based on average performance from internal testing	≥85% (for exome panel)*	>80%
Specialized Sample Types	Blood, Saliva	Blood, FFPE Tissue, Saliva	FFPE Tissue
Species Category	Human	Human, Other	Human
System Compatibility	HiSeq 3000, HiSeq 4000, NextSeq 1000, NextSeq 2000, NextSeq 550, NextSeq 550Dx in Research Mode, NovaSeq 6000	HiSeq 3000, HiSeq 4000, iSeq 100, MiniSeq, MiSeq, MiSeqDx in Research Mode, NextSeq 2000, NextSeq 550, NextSeq 550Dx in Research Mode, NovaSeq 6000	HiSeq 3000, HiSeq 4000, NextSeq 1000, NextSeq 2000, NextSeq 500, NextSeq 550, NovaSeq 6000
Technology	Sequencing	Sequencing	Sequencing
Uniformity	≥ 91% (% coverage at 20× for 5 Gb) based on average performance from internal testing	≥90% (% coverage at 20x for 4 Gb; for exome panel)	>85% (% coverage at 20x for 4 Gb)
Variant Class	Copy Number Variants (CNVs), Germline Variants, Insertions-Deletions (indels), Loss of Heterozygosity (LOH), Single Nucleotide Polymorphisms (SNPs), Somatic Variants	Copy Number Variants (CNVs), Germline Variants, Insertions-Deletions (indels), Loss of Heterozygosity (LOH), Single Nucleotide Polymorphisms (SNPs), Somatic Variants

Method-Specific Workflow

Prepare Library with:

Illumina DNA Prep with Exome 2.0 Plus Enrichment

Sequence Efficiently with:

Analyze Your Data with:

Key Performance using DRAGEN Analysis

High performance of Illumina DNA Prep with Exome 2.0 Plus Enrichment which is improved compared to the original Illumina Exome Panel. Libraries were constructed and enriched using Illumina DNA Prep with Exome 2.0 Plus kit with either the included Exome 2.0 Plus Panel or the Illumina Exome Panel (IEP, 20020183) with hybridization (1.5 or 16 hr, 1.5 hr for Illumina Exome Panel) and washing temperatures of 62°C for Exome 2.0 Plus or 58°C for Illumina Exome Panel. Enriched libraries were sequenced on a NovaSeq 6000/S4 flow cell using 2 x 101 bp chemistry, and 50M reads per enriched library were analyzed using DRAGEN and the average results presented.

In Silico and Experimental Coverage of Key Public Databases

Experimental On-Target Rates vs. Public Databases

High database target coverage rates both in silico and experimentally. The bed file for the Exome 2.0 Plus Panel was compared to the listed public databases and the percent overlap was determined and reported. For the experimental data, libraries were constructed and enriched using the Illumina DNA Prep with Exome 2.0 Plus with Exome 2.0 Plus kit using a 1.5 hr (orange) or 16-hour (blue) hybridization time and washing temperatures of 62°C. Enriched libraries were sequenced on a NovaSeq 6000/S4 flow cell with 2 x 101 bp chemistry, and 50M reads per enriched library were analyzed using DRAGEN and the average results presented.

Product Literature

Data Sheet | PDF | 7 versions

Manuals and Support Information

Custom Protocol Selector
Generates customized, end-to-end instructions

Illumina DNA Prep with Exome 2.0 Plus Enrichment Support Documentation

NovaSeq 6000 Reagent Kits

Reagent kits for the NovaSeq 6000 system provide ready-to-use cartridge-based reagents for cluster generation and SBS.

NextSeq 1000/2000 Reagents

NextSeq 1000/2000 reagent kits contain a reagent cartridge, flow cell, and resuspension buffer. Multiple flow cell configurations offer adjustable output based on project needs.

NextSeq 500/550 v2.5 Kits

These sequencing reagent kits offer increased stability and robustness over the v2 kit version.

Illumina DRAGEN Secondary Analysis

The Illumina DRAGEN Secondary Analysis provides accurate, comprehensive, and efficient analysis of next-generation sequencing data.

Clarity LIMS

Clarity LIMS is a laboratory information management system designed for genomics labs to track samples and manage workflows for an optimized and efficient lab.

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