The AmpliSeq for Illumina Cancer Hotspot Panel v2 is a targeted resequencing assay for researching somatic mutations across the hotspot regions of 50 genes with known associations to cancer, as identified in the Catalogue of Somatic Mutations in Cancer (COSMIC)1 database. Key features include:
The Cancer HotSpot Panel is part of an integrated workflow that includes AmpliSeq for Illumina polymerase chain reaction (PCR)-based library preparation, Illumina sequencing by synthesis (SBS) next-generation sequencing (NGS) technology, and automated analysis.
This panel enables highly sensitive variant detection within multiple cancer types, including lung, colon, breast, ovarian, melanoma, and prostate. The ready-to-use panel saves you the time and effort of identifying targets, designing primers, and optimizing panels.
Instrument | Recommended Number of Samples | Read Length |
---|---|---|
iSeq 100 System | 16 samples per run (assumes minimum coverage of 500×) | 2 × 150 bp |
MiniSeq System | Samples per run: mid output: 32, high output: 96 (assumes minimum coverage of 500×) | 2 × 150 bp |
MiSeq System | Samples per run (by reagent kit version): v2 nano: 4, v2 micro: 16, v2: 60, v3: 96 (assumes minimum coverage of 500×) | 2 × 150 bp |
AmpliSeq for Illumina Cancer Hotspot Panel v2 | AmpliSeq for Illumina Focus Panel | AmpliSeq for Illumina Comprehensive Panel v3 | TruSight Tumor 15 | |
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Assay Time | 5 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 5-6 hours (library prep only; doesn't include library quantification, normalization, or pooling time) | 7 hours |
Cancer Type | Pan-Cancer, Solid Tumor | Solid Tumor | Solid Tumor | Solid Tumor |
Content Specifications | Hotspot regions of 50 genes with known associations to cancer | DNA and RNA targets for 52 oncogenes | DNA and RNA targets for 161 oncogenes | Amplifies 250 amplicons from 15 genes associated with solid tumors |
Description | Somatic analysis research into hotspot regions of 50 cancer-related genes. | Somatic analysis research on 52 genes associated with solid tumor cancers. | Somatic analysis research on hotspot and full-length targets of genes* associated with solid tumor cancers. | Focused panel to study relevant solid tumor somatic variants in 15 genes using a simple workflow. |
Input Quantity | 1–100 ng (10 ng recommended per pool) | 1–100 ng (10 ng recommended per pool) | 1–100 ng (10 ng recommended per pool) | 20 ng |
Method | Amplicon Sequencing, Targeted DNA Sequencing | Amplicon Sequencing, Targeted DNA Sequencing, Targeted RNA Sequencing | Amplicon Sequencing, Targeted DNA Sequencing, Targeted RNA Sequencing | Amplicon Sequencing, Targeted DNA Sequencing |
Multiplexing | 96 dual index combinations | 96 dual index combinations | 96 dual index combinations | 1-24-plex |
Nucleic Acid Type | DNA | DNA, RNA | DNA, RNA | DNA |
Specialized Sample Types | FFPE Tissue | FFPE Tissue | FFPE Tissue | FFPE Tissue |
Species Category | Human | Human | Human | Human |
AmpliSeq for Illumina Oncology Panels on the iSeq 100 System
Application Note | PDF < 1 MB
Automated Solutions for AmpliSeq for Illumina Sequencing Panels
Application Note | HTML
AmpliSeq for Illumina Cancer HotSpot Panel Gene List
product_file | EXCEL < 1 MB
Data Sheet | HTML | 5 versions
Sample identification with the AmpliSeq for Illumina Sample ID Panel
Application Note | PDF < 1 MB
Illumina Adapter Sequences Documentation
AmpliSeq for Illumina Cancer HotSpot Panel v2 Checklist Documentation
AmpliSeq for Illumina Cancer HotSpot Panel v2 Reference Guide Documentation
AmpliSeq for Illumina Direct FFPE DNA Kit Reference Guide Documentation
AmpliSeq for Illumina Cancer HotSpot Panel v2 Consumables & Equipment Documentation
AmpliSeq for Illumina Cancer Hotspot Panel v2 Documentation
Index Adapters Pooling Guide Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions