Targeted methylation sequencing (Methyl-Seq) offers a balanced, cost-effective choice between whole-genome bisulfite sequencing and methylation arrays that can support both screening and biomarker discovery study objectives.1 TruSeq Methyl Capture EPIC Library Prep supports researchers seeking a deeper understanding of the role of methylation in gene regulation.
Comprehensive methylation-site content: Offers significantly higher coverage of emerging epigenetic regions of interest compared to other Methyl-Seq kits currently on the market (See Supporting Data section, Epigenetic Regions Covered).
Maximize discovery power, minimize cost: Efficiently query differentially methylated bases to conserve research dollars.
Convenient kit configuration: Includes bisulfite conversion, library prep, target enrichment, and purification reagents in a single kit for hassle-free ordering.
User-friendly data analysis: Analyze sequence data in BaseSpace Sequence Hub with methylation apps designed for biologists.
| Instrument | Recommended Number of Samples | Read Length |
|---|---|---|
| NextSeq 550 System | 8 samples per run (high output; based on 40× mean coverage and >90% of target bases covered at ≥10×) | 2 x 100 bp (max recommended) |
| NovaSeq 6000 System | Samples per run (dual flow cell): S1: 48, S2: 48, S4: 96 (limited by available index combinations; based on >55 million reads) | 2 × 100 bp (max recommended) |
| HiSeq 3000 System | 48 samples per run (based on 40× mean coverage and >90% of target bases covered at ≥10×) | 2 × 100 bp (max recommended) |
| HiSeq 4000 System | 96 samples per run (based on 40× mean coverage and >90% of target bases covered at ≥10×) | 2 × 100 bp (max recommended) |
| HiSeq 4000 System | 96 samples per run (based on 40× mean coverage and >90% of target bases covered at ≥10×) | 2 × 100 bp (max recommended) |
| TruSeq Methyl Capture EPIC Library Prep Kit | Infinium MethylationEPIC v2.0 Kit | |
|---|---|---|
| Content Specifications | Targets over 3.3 million CpGs. Interrogates biologically important methylome targets. | |
| Description | Focused interrogation of a set of methylome targets for a balanced, cost-effective option that can support both screening studies and biomarker discovery research objectives. | Quantitative interrogation of selected methylation sites across the genome, offering high-throughput research capabilities at a minimal cost per sample. |
| Input Quantity | 500 ng human genomic DNA | 250 ng DNA |
| Method | Methylation Sequencing, Targeted DNA Sequencing | Methylation Array |
| Number of Samples | Up to 12-plex | 8 samples per array |
| Species Category | Human | Human |
| Technology | Sequencing | Microarray |
The TruSeq Methyl Capture EPIC Library Prep Kit uniquely targets emerging epigenetic regions of interest such as open chromatin, ENCODE, and FANTOM regions. The percentage of genomic regions covered using the TruSeq Methyl Capture EPIC Library Prep Kit, the Competitor X Methyl-Seq Kit2 and the Competitor Y Methyl-Seq Kit3 are shown.
TruSeq Methyl Capture EPIC Library Prep Kit
Data Sheet | PDF 2 MB
Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in as little as 90 minutes, with a low DNA input requirement.
Robust methylation profiling microarray with extensive coverage of CpG islands, genes, and enhancers. Use for epigenome-wide association studies.
Targeted panel that measures expression levels of >20,000 human RefSeq genes.
